Anticonvulsant and sedative activities of aqueous leave extract of Leucas martinicensis (Jacq.) R. Br

Leucas martinicensis is a medicinal plant used in traditional medicine to treat convulsions and epilepsy. The present study was to evaluate the anticonvulsant and sedative effects of the aqueous leave extract of L. martinicensis in Wistar rats. The anticonvulsant activities of L. martinicensis (50, 100, 200 or 400 mg/kg i.p.) were evaluated using maximal electroshock seizure (MES) - and strychnine (STR) -induced seizure models while the sedative properties were evaluated using the diazepaminduced sleep model in Wistar rats. The 400 mg/kg of the extract protected rats (100%) against seizures in both models while at 200 mg/kg seizure protection (100%) was only in STR model. There was a significant (p<0.05) delay in the onset and reduction in the duration of seizure in the two models in unprotected rats. L. martinicensis exerted sedative effect by significantly reducing the onset (sleep latency) and increasing the total duration of sleep induced by diazepam. These results suggest that aqueous extract of L. martinicensis may possess anticonvulsant and sedative properties that might show efficacy against primary generalised seizures and secondarily generalised tonic -clonic seizures in humans. It also lends pharmacological credence to the use of the plant in traditional medicine for the management of epilepsy and convulsions.Keywords: Leucas martinicensis; Epilepsy; Traditional medicine; Anticonvulsant; slee

Isolation of oleanolic acid from Parinari curatellifolia (Planch Ex. Benth) stem bark and evaluation of its anticonvulsant and sedative activities in rodents

Parinari curatellifolia is used by traditional medicine practitioners for the treatment of epilepsy. So far, no study has isolated the active principle that may be responsible for its anticonvulsant activity. The study aimed to isolate compound(s) present from Parinari curatellifolia that may be responsible for its anticonvulsant activity. The ethyl acetate fraction of the stem bark of Parinari curatellifolia was chromatographed over silica gel column chromatography which led to the isolation of compound C. The structure of the compound was elucidated using IR, 1H-NMR, 13CNMR and DEPT-135 spectroscopy. Acute toxicity study of the isolated compound was evaluated in mice using OECD 425 guidelines (2000 mg/kg orally). The anticonvulsant study of the isolated compound (at 50, 75 and 100 mg/kg) was evaluated in mice using pentylenetetrazole (PTZ)- induced convulsion. The sedative properties of the compound (at 10, 50 and 100 mg/kg) were evaluated using the diazepam-induced sleep model in rats. Structure elucidation of the isolated compound confirmed the compound to be oleanolic acid. Acute toxicity study revealed no lethal effects at 2000 mg/kg. the compound (oleanolic acid) significantly (p<0.05) increased the onset of seizure at all doses and resulted in 25% protection against seizure at 100 mg/kg. It exerted sedative effect at all doses by significantly (p<0.05) reducing sleep latency and increasing total duration of sleep induced by diazepam. The results obtained from this study have revealed the presence of oleanolic acid in P. curatellifolia and have shown its anticonvulsant and sedative activities for the first time.The authors expressed their sincere appreciation to the Academic Staff Union of Universities (ASUU), Tertiary Education Trust fund (TETFund) and the management of Usmanu Danfodiyo University Sokoto for their financial support.The Academic Staff Union of Universities (ASUU), Tertiary Education Trust fund (TETFund) and the management of Usmanu Danfodiyo University Sokoto.https://www.tjnpr.orgam2020Chemistr

Ficus platyphylla promotes fertility in female Rattus norvegicus Wistar strain: a preliminary study

<p>Abstract</p> <p>Background</p> <p><it>Ficus platyphylla </it>Delile (family- Moracea) commonly called gutta percha tree is a deciduous plant found in savannah areas. It grows widely in the Northern part of Nigeria, up to 60 ft. high and is known as 'gamji' by the Hausas. The seeds, bark and leaves have been used traditionally in combination to promote fertility. Scientifically, the plant has been shown to have analgesic, anti-inflammatory and CNS effects. The present study was to validate the use of this plant to promote fertility in female Rattus norvegicus Wistar strain using various fertility parameters.</p> <p>Methods</p> <p>Female Rattus norvegicus Wistar strain weighing between 150-180 g were randomly selected and divided into two major groups. Each group was subdivided into 5 treatment groups of 100, 200, 400 mg/kg BW of aqueous extract of <it>F. platyphylla </it>and a control group of 5 ml/kg of distilled water. A positive control of clomiphene citrate was used. Treatment of the first group was discontinued after 15 days prior to mating (pre-mating treatment group), while the other was treated continuously till delivery (continuous treatment group). At the 10^thday, females were sacrificed and implantation sites were checked and embryos counted. Upon delivery, litter sizes were determined and the pups weighed and checked for deformities. Other reproductive indices were calculated. Data were analyzed by one-way analysis of variance and students T-test. Proportions were analysed by Chi square. Statistical evaluations were performed using STATS programs and Graphpad prism, and a difference was considered statistically significant at P < 0.05.</p> <p>Results</p> <p>There was a significant reduction in the percentage post implantation losses of both the pre-treatment and the continuous treatment groups when compared to their distil water controls. The litter size of the pre-treatment group was similar to the distil water group while at 400 mg/kg, the continuous treatment group showed an increase in the litter size similar to that of the clomiphene group. There were no observed external deformities in the pups.</p> <p>Conclusions</p> <p>Administration of aqueous extract of <it>F. platyphylla </it>promotes fertility by reducing post implantation loss and by increasing litter size in female Rattus norvegicus Wistar strain.</p

Neuropharmacological evaluation of the methanol leaf extract of Phyllanthus muellerianus (Kuntze) Exell and its ethyl acetate fraction in mice

Purpose: To investigate the neuropharmacological effects of the methanol leaf extract (ME) and fractions of Phyllanthus muellerianus (PM) (Phyllanthaceae) (Kuntze) Exell (PM) in mice. Methods: Acute toxicity was carried out on the extract using standard protocol. ME was fractionated into hexane (HF), ethyl acetate (EF), and methanol (MF) fractions. Pentylenetetrazol (PTZ)-induced seizure, open field (OF) and motor coordination (rotarod) tests were models employed. Mice allotted into fourteen groups of six animals each were treated orally with 100, 200, or 400 mg/kg of the extract and fractions in pentylene tetrazole (PTZ) seizure model. Seizure was induced with intraperitoneal (ip) injection of 70 mg/kg of PTZ. The positive and negative controls employed were phenobarbitone (35 mg/kg) and 5 ml/kg of 7 % Tween 80, respectively. In the OF and motor coordination tests, six groups of six mice were treated orally with ME and EF at 200 and 400 mg/kg doses. Control groups received either 5 ml/kg of 7% Tween 80 or diazepam (1 mg/kg ip) as negative and positive controls respectively Results: In the PTZ model, only EF abolished seizures completely (p&lt;0.05), when compared with the negative control, producing 100% protection, even better than the phenobarbitone which gave 83.3% protection. In the OFT, in comparison with the control, ME at 400 mg/kg (p &lt; 0.05) decreased both the number of line crossing and the number of assisted rearing similar to that produced by diazepam. EF increased both the locomotor and exploratory activities significantly (p &lt; 0.05) in mice. ME at 400 mg/kg significantly (p &lt; 0.05) evoked reduction in the time of fall of mice from the rotarod when compared to the control in the same way as diazepam while EF did not elicit any appreciable differences. Conclusion: ME has anticonvulsant, sedative, and anxiolytic activities, while EF possesses anticonvulsant and anxiolytic activities devoid of sedative and cognitive impairment. The observed anticonvulsant effect was better than that produced by phenobarbitone. Thus, it may be a good lead for developing antiepileptic and other central nervous system active agents